RNA-seq based transcriptomic map reveals multiple pathways of necroptosis in treating myocardial ischemia reperfusion injury.

Necroptosis has been shown to contribute to myocardial ischemia reperfusion injury (MIRI). This study aims to gain new insights into the signaling pathway of necroptosis in rat MIRI using RNA sequencing. MIRI was induced in male rats by ligating the left anterior descending coronary artery for 30 min, followed by reperfusion for 120 min. RNA sequencing was performed to obtain mRNA profiles of MIRI group and MIRI group treated with necrostatin-1 (Nec-1,an inhibitor of necroptosis). Differentially expressed genes (DEGs) were then identified. The DEGs were prominently enriched in the TNF-α signaling pathway, the MAPK signaling pathway and cytokine-cytokine receptor pathways. The majority of the results were associated with genes like Thumpd3,Egr2,Dot1l,Cyp1a1,Dbnl,which primarily regulate inflammatory response and apoptosis, particularly in myocardium. The above results suggested that Nec-1 might be involved in the regulation of necroptosis and the inflammatory response through the above-mentioned genes.

Clinical analysis of renal cell carcinoma with intestinal metastasis resulting in small bowel and colon fistula.

An enteric fistula is an abnormal connection between the intestine and other organs and is often caused by inflammation or trauma. Diagnosis and treatment involve imaging and endoscopy. Treatment may include medication and surgery. This report presents a rare case of severe enteric fistula caused by colonic metastasis of clear cell renal carcinoma. The objective of this report is to increase surgeons' awareness of atypical manifestations of intestinal metastatic cancer.